The
View from Here
William S. Marshall
This Editors Choice focuses on the use of RNA interference (RNAi) for target
identification and validation. RNAi denotes a set of post-transcriptional gene
regulatory phenomena mediated by small noncoding RNA's that appear to play a
fundamental role in biology...
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more...
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Biology
calls the targets: combining RNAi and disease biology
Helmuth
H. G. van Es and Gert-Jan Arts
Drug Discovery Today
Target-based
drug discovery starts with the identification of target genes and
their respective protein products (associated with or controlling
a disease-relevant phenotype) that, when inhibited or activated,
ameliorate the associated disease. To identify disease-relevant
genes, robust tools are needed to allow biology-driven target discovery
and validation...
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Exploring
the sounds of silence: RNAi-mediated gene silencing for target identification
and validation
Moitreyee
Chatterjee-Kishore and Christopher P. Miller
Drug Discovery Today
Drug development begins with the identification and early preclinical
validation of novel biological targets, a process often called ‘target
identification and validation’. This process usually uses
various approaches, such as observations from literature and findings
from animal or clinical studies, together with cutting edge molecular
techniques that include analyses of gene and protein expression,
interaction and function...
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Harnessing
in vivo siRNA delivery for drug discovery and therapeutic
development
Frank Y. Xie, Martin C. Woodle and Patrick Y. Lu
Drug Discovery Today
The use of RNA interference (RNAi) is spreading rapidly to nearly
every aspect of biomedical research. The gene silencing capability
of RNAi is being used to study individual gene’s biological
function and role in biochemical pathways. However, the efficacy
of RNAi depends upon efficient delivery of the intermediates of
RNAi, short interfering RNA (siRNA) and short hairpin RNA (shRNA)
oligonucleotides...
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Abstracts of Key Research Articles
Position-specific
chemical modification of siRNAs reduces "off-target"
transcript silencing
Jackson AL
et al.
RNA. 2006 Jul;12(7):1197-205
Fatality
in mice due to oversaturation of cellular microRNA/short hairpin
RNA pathways
Grimm D et al.
Nature. 2006 May 25;441(7092):537-41
3'
UTR seed matches, but not overall identity, are associated with
RNAi off-targets
Birmingham A et al.
Nat Methods. 2006 Mar;3(3):199-204
RNAi-mediated
gene silencing in non-human primates
Zimmermann TS et
al.
Nature. 2006 May 4;441(7089):111-4
High-throughput
RNAi screening in cultured cells: a user's guide
Echeverri CJ, Perrimon N.
Nat Rev Genet. 2006 May;7(5):373-84
Passenger-strand
cleavage facilitates assembly of siRNA into Ago2-containing RNAi
enzyme complexes
Matranga C et al.
Cell. 2005 Nov 18;123(4):607-20
The
widespread impact of mammalian MicroRNAs on mRNA repression and
evolution
Farh KK et
al.
Science. 2005 Dec 16;310(5755):1817-21
Genome-wide
RNAi analysis of growth and viability in Drosophila cells
Boutros M et al.
Science. 2004 Feb 6;303(5659):832-5
A
large-scale RNAi screen in human cells identifies new components
of the p53 pathway
Berns K et
al.
Nature. 2004 Mar 25;428(6981):431-7
Rational
siRNA design for RNA interference
Reynolds A et
al.
Nat Biotechnol. 2004 Mar;22(3):326-30 |
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